Genox

‘New 8-OHdG Check’ ELISA Kit
(ELISA kit for 8-hydroxy-2’-deoxyguanosine)
(Catalog #KOG-200SE)

Product Insert (pdf)


8-OHdG Check is a competitive in vitro enzyme-linked immuno-sorbent assay for quantitative measurement of the oxidative DNA adduct, 8-hydroxy-2'-deoxyguanosine
(8-OHdG)
in samples expected to have high levels of 8-OHdG, such as the urine.

Features of the ‘New 8-OHdG Check’ Elisa Kit

1.

Easy operation:

There is no need for expensive equipments and sample pretreatment.

2.

Highly specific:

Monoclonal antibody recognizes 8-OHdG specifically
(21 analogues of 8-OHdG do not have any cross reactivity).

3.

Speed:

Estimated time of assay is 3.5 to 4 hours.

4.

With blank and standard 24 samples can be tested in triplicate. Only 150µl
of sample is required (50µl per well).

5.

The use of split type microplate prevents the waste of reagents.


Merits of the ‘New 8-OHdG Check’ Elisa Kit

The '8-OHdG Check' is useful as a non-invasive indicator in numerous ways, such as:

  • Use as a screening protocol to learn the role of oxidative stress biomarker 8-OHdG in aging and for application of this biomarker in the advancement of basic and applied research in biology and medicine.
  • In nutrition, diet, and life style related basic and applied research.
  • In evaluating the extent of oxidative stress generated as a result of exercises performed by the body or specific actions to which the body is subjected to.
  • In determining the side effects of oxidative stress following clinical procedures
    or intake of  therapeutic drugs.


Kit Contents

  • 8-OHdG pre-coated microtiter plate (split type)
  • Primary antibody (Anti-8-OHdG monoclonal antibody)
  • Primary antibody reconstituting buffer
  • Secondary antibody (HRP-conjugated anti mouse antibody)
  • Secondary antibody reconstituting buffer
  • Chromatic solution (3,3',5,5'-tetramethylbenzidine)
  • Diluting solution
  • Washing solution
  • Reaction terminating solution
  • Standard 8-OHdG solution (0.5, 2, 8, 20, 80, 200 ng/ml)
  • Plate seal


Assay Procedure

(1)  Primary Antibody Reaction (Competitive Reaction): 37°C for 1hour
(2)  Secondary Antibody Reaction: 37°C for 1hour
(3)  Development of Color Reaction: Room Temperature for 15min in the dark
(4)  Absorbance Reading (wavelength at 450 nm) and Calculation of Results


REFERENCES

  1. T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
    Induction and nuclear translocation of thioredoxin by oxidative damage in the
    mouse kidney: independence of tubular necrosis and sulfhydryl depletion.
    Lab. Invest. 77(2), p145-155 (1997).
  2. Y.Hattori, C.Nishigori, T.Tanaka, K.Uchida, O.Nikaido, T.Osawa, H.Hiai, S.Imamura, and S.Toyokuni:
    8-Hydroxy-2'deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure.
    J. Invest. Dermatol. 107, p733-737 (1997).
  3. S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi, and T.Osawa:
    Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine
    by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model.
    Lab. Invest. 76(3), p365-374 (1997).
  4. S.S.Kantha, S.Wada, H.Tanaka, M.Takeuchi, S.Watabe, and H.Ochi
    Carnosine sustains the retention of cell morphology in continuous fibroblast culture subjected to nutritional insult.
    Biochemical and Biophysical Research Communications, 223, 278-282 (1996)
  5. S.S.Kantha, S.Wada, M.Takeuchi, S.Watabe, and H.Ochi
    A sensitive method to screen for hydroxyl radical scavenging activity in natural food extracts using competitive inhibition ELISA for 8-hydroxydeoxyguanosine.
    Biotechnology Techniques, 10(12), 933-936 (1996)  
  6. J.Leinonen, T.Lehtimaki, S.Toyokuni, K.Okada, T.Tanaka, H.Hiai, H.Ochi,
    P.Laippala, V.Rantalaiho, O.Wirta, A.Pasternack, and H.Alho
    New biomarker evidence of oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus.
    FEBS Letters, 417, 150-152 (1997)  
  7. S.Takahashi, M.Hirose, S.Tamano, M.Ozaki, S.Orita, M.Takeuchi, H.Ochi, S.Fukuda, H.Kasai, and T.Shirai:
    Immunohistochemical detection of 8-hydroxy-2ÅEdeoxyguanosine in paraffin-embedded sections of rat liver after carbon tetrachloride treatment.
    Toxicol. Pathol. 26, p247-252 (1998).
  8. H.Tsuboi, K.Kouda, H.Takeuchi, M.Takigawa, Y.Masamoto, M.Takeuchi, and H.Ochi
    8-Hydroxydeoxyguanosine in urine as an index of oxidative damage to DNA in the evaluation of atopic dermatitis.
    British Journal of Dermatology, 138, 1033-1035 (1998)  
  9. Y.Miyake, K.Yamamoto, N.Tsujihara, and T.Osawa
    Protective effects of lemon flavonoids on oxidative stress in diabetic rats.
    Lipids, 33(7), 689-695 (1998)  
  10. M-H.Kang, M.Naito, N.Tsujihara, and T.Osawa
    Sesamolin inhibits lipid peroxidation in rat liver and kidney.
    Journal of Nutrition, 128, 1018-1022 (1998)  
  11. N.U.Ahmed, M.Ueda, O.Nikaido, T.Osawa and M.Ichihashi:
    High levels of 8-hydroxy-2'deoxyguanosine appear in normal human epidermis
    after a singer dose of ultraviolet radiation.
    Br. J. Dermatol. 140, p226-231 (1999).
  12. Y.Ihara, S.Toyokuni, K.Uchida, H.Odaka, T.Tanaka, H.Ikeda, H.Hiai, Y.Seino, and Y.Yamada:
    Hyperglycemia causes oxidative stress in pancreatic -cells of GK rats, a model of type 2 diabetes.
    Diabetes 48, p927-932 (1999).
  13. S.Kondo, S.Toyokuni, Y.Iwasa, T.Tanaka, H.Onodera, H.Hiai and M.Imamura:
    Persistent oxidative stress in human colorectal carcinoma, but not in adenoma.
    Free Rad. Biol. Med. 27, p401-410 (1999).
  14. S.Toyokuni, Y.Iwasa, S.Kondo, T.Tanaka, H.Ochi, and H.Hiai:
    Intranuclear distribution of 8-hydroxy-2'-deoxyguanosine: An immunocytochemical study.
    J. Histochem. Cytochem. 47(6), p833-835 (1999).
  15. S.Toyokuni:
    Reactive oxygen species-induced molecular damage and its application in pathology.
    Pathol. Internat. 49, p91-102 (1999).
  16. T.Arimoto, T.Yoshikawa, H.Takano, M.Kohno
    Generation of reactive oxygen species and 8-hydroxy-2'-deoxyguanosine
    formation from diesel exhaust particles components in L1210 cells.
    Japanese Journal of Pharmacology, 80, 49-54 (1999)  
  17. M.D.Evans, M.S.Cooke, I.D.Podmore, Q.Zheng, K.E.Herbert, and J.Lunec
    Discrepancies in the measurement of UVC-induced 8-oxo-2'-deoxyguanosine: Implications for the analysis of oxidative DNA damage.
    Biochemical and Biophysical Research Communications, 259, 374-378 (1999)     
  18. S.Toyokuni:
    An in vitro Feton reaction model and the application of immunohistochemistry to detect oxidative damage.
    Models and Methods in Cell Signaling and Gene Expression: Applications to Oxidative Stress Research., Chapter four,  pp40-54 (2000).
  19. K.Yamagami, Y.Yamamoto, M.Kume, Y.Ishikawa, Y.Yamaoka, H.Hiai and S.Toyokuni:
    Formation of 8-hydroxy-2'deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in rat liver after ischemia-reperfusion: Distinct localization of the two oxidatively modified products.   Antioxidants & Redox Signaling, 2(1), p127-136 (2000).
  20. D.Nakae, H.Akai, H.Kishida, O.Kusuoka, M.Tsutsumi, and Y.Konishi:
    Age and organ dependent spontaneous generation of nuclear 8-hydroxydeoxyguanosine in male Fischer 344 rats.
    Lab. Invest. 80(2), p249-261 (2000).
  21. S.Liardet, C.Scaletta, R.Panizzon, P.Hohlfeld and L.L.Applegate:
    Protection against pyrimidine dimmers, p53, and 8-hydroxy-2'deoxyguanosine expression in ultraviolet-irradiated human skin by sunscreens: difference between UVB + UVA and UVB alone sunscreens.
    J. Investigative Dermatol. 117(6), p1437-1441 (2001)
The 'New 8-OHdG Check ELISA Kit is manufactured in Japan by the Japan Institute for the Control of Aging (JaICA). Genox is the distributor of this kit for users in North and South America.

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Users of Genox’s products are strongly advised NOT TO USE THEM FOR CLINICAL/DIAGNOSTIC APPLICATIONS. 

The provision of test reports, generated by using Genox's products, to individuals
or to treating physicians for the diagnosis, prevention, treatment and control of any human disease or impairment of, or the assessment of the health, nutritional, or medical condition of individuals is expressly prohibited by law.
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