Genox

Anti-Methylglyoxal Monoclonal Antibody
(Anti MG monoclonal antibody, specific for argpyrimidine, clone #3C)
(Catalog #MMG-030 for 30 microgram vial)

Product Insert (pdf)pdf

What is Methylglyoxal ?
Methylglyoxal (MG), an endogenous metabolite that increases in diabetes and a common intermediate in the Maillard reaction (glycation), reacts with proteins and forms advanced glycation end products (AGE). MG reacts with arginine residue in protein and forms numerous numbers of adducts, such as argpyrimidine. This antibody is specific for argpyrimidine: N-(5-hydroxy-4,6-dimethylpyrimidine-2-yl)-L-ornithine.

mono2a

 

Clone #

:

3C

Antigen

:

MG-modified keyhole-lympet hemocyanine

Specificity

:

Specific for MG-modified protein (especially Argpyrimidine)

Subclass

:

Mouse IgG2a

Form

:

Protein A purified.
Frozen (10mM PBS containing 0.1 %NaN3 and 0.5% BSA).
100 micro g/mL IgG.

Application

:

Immunohistochemistry?It is recommended that the antibody
be tried at 0.5-1.0 micro g/mL on paraformaldehyde fixed tissue

Storage

:

Less than -20°C

Specificity of anti MG antibody.

 


Immunohistochemical detection of MG adducts in atherosclerotic aorta.
Noriyuki Shibata et al. Tokyo Women’s medical University

 

IMMUNOHISTOCHEMISTRY
Procedure
1)

Paraffin sections were deparaffinized and rehydrated.

2)

Sections were quenched for several minutes with 3% hydrogen
peroxide, rinsed in PBS, pretreated for 30 min with 3% nonimmune
animal serum in PBS.

3)

Antigen retrieval by microwave treatment.

4)

Sections were incubated overnight at 4°C with a antibody
at a concentration of 0.5-10 micro gram/mL.

5)

Antibody binding was visualized by the avidin-biotin-immuno peroxidase
complex method using the Vectastain ABC kit (Vector, Burlingame, CA)
according to the manufacturer's instructions.

6)

3,3-Diaminobenzidine tetrahydrochloride was used as the chromogen.

7)

Hematoxylin or Eosin was used as the counter stain.

 

 

Technical Note
1)

Sections from which the primary antibodies were omitted served
as negative reaction controls.

2)

For frozen materials, samples were fixed in 10% formalin, immersed
in 30% sucrose in PBS, embedded at OCT (Sakura, Tokyo, Japan),
and stored at 80°C.

3)

For paraffin embedded materials, samples were fixed in 10% formalin, dehydrated, embedded in paraffin, and stored at room temperature.

4)

Microwave treatment improves the target staining, whereas it declines
non-specific background.

5)

Protease K treatment declines the target staining.

Positive control
Aortic wall in the renal tissue of diabetic nephropathy or aortic wall of atherosclerosis.

REFERENCES

1)

Protein modification by a Maillard reaction intermediate methylglyoxal. Immunochemical detection of fluorescent 5-methylimidazolone derivatives in vivo.
Uchida K, Khor OT, Oya T, Osawa T, Yasuda Y, Miyata T
FEBS Lett. 410(2-3), p313-318. (1997)

2)

Methylglyoxal Modification of Protein -Chemical and Immunochemical Characterization of Methylglyoxal-Arginine Adduct.
T.Oya, N.Hattori, Y.Mizuno, S.Miyata, S.Maeda, T.Osawa and K.Uchida
J.Biological Chemistry, 274, 18492-18502 (1999)

3)

Advanced glycation and lipidoxidation of the peritoneal membrane: respective roles of serum and peritoneal fluid reactive carbonyl compounds.
Miyata T, Horie K, Ueda Y, Fujita Y, Izuhara Y, Hirano H, Uchida K, Saito A, van Ypersele de Strihou C, Kurokawa K
Kidney Int. 58(1), p425-435.(2000)

4)

Argpyrimidine, a blue fluorophore in human lens proteins: high levels in brunescent cataractous lenses.
Padayatti PS, Ng AS, Uchida K, Glomb MA, Nagaraj RH
Invest Ophthalmol Vis Sci. 42(6), p1299-1304. (2001)

5)

High concentrations of glucose induce synthesis of argpyrimidine in retinal endothelial cells.
Padayatti PS, Jiang C, Glomb MA, Uchida K, Nagaraj RH
Curr Eye Res. 23(2), p106-115. (2001)

6)

Curcumin Inhibits ROS Formation and Apoptosis in Methylglyoxal- Treated Human Hepatoma G2 Cells.
WEN-HSIUNG CHAN, HSIN-JUNG WU and YAN-DER HSUUW
Ann. N.Y. Acad. Sci. 1042: 372-378 (2005)

The ‘Anti-MMG Monoclonal Antibody’ is manufactured in Japan
by the Life Science Products Division of the Nippon Oils and Fats
Corporation, Japan. Genox is the distributor of this product
for users in North and South America.

ORDER THIS PRODUCT

Note: Genox’s Products are for RESEARCH USE ONLY.
Users of Genox’s products are strongly advised NOT TO USE THEM FOR CLINICAL/DIAGNOSTIC APPLICATIONS. 

The provision of test reports, generated by using Genox's products, to individuals
or to treating physicians for the diagnosis, prevention, treatment and control of any human disease or impairment of, or the assessment of the health, nutritional, or medical condition of individuals is expressly prohibited by law.
  42 U.S.C. § 263a (2006); 42 C.F.R. § 493.3 (2006); COMAR 10.10.01.02 (2006). Genox is not a CLIA certified laboratory.

© Copyright 1996-2016 Genox Corporation. All rights reserved.

ELISA Testing Kits
Genox Corporation has
developed biomarker
assays to measure
several oxidative
damage biomarkers.
upcoming events