Genox

Anti-Acrolein Monoclonal Antibody
(Anti Acrolein monoclonal antibody, clone #F56)
 (Catalog #MACR-100 for 100 microgram vial)
(Catalog #MACR-030 for 30 microgram vial)

Product Insert (pdf)pdf

What is Acrolein?
Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidase catalyzed amino acid oxidation. ACR is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with ACR and lysine residue leads to the formation of numerous numbers of adducts, such as formyl-dehydropiperidino-lysine (FDP-lysine) type derivative. This antibody is specific for the ACR modified protein, especially FDP-lysine type derivative.

mono2a

 

Clone #

:

5F6

Antigen

:

ACR-modified keyhole-lympet hemocyanine

Form

:

Frozen (100 micro g/mL antibody in 10mM PBS containing 0.1 %NaN3 and 0.5% BSA). Purified by Protein-A.

Application

:

Immunohistochemistry.
Recommended antibody concentration is 0.5-1.0 micro gram/mL on paraformaldehyde fixed tissue.

Specificity

:

Specific for ACR-modified protein (especially FDP-lysine type derivative)

Subclass

:

Mouse IgG1,kappa

Storage

:

Less than -20°C



Specificity of anti ACR antibody.

 


Immunohistochemical detection of MG adducts in atherosclerotic aorta.
Noriyuki Shibata et al. Tokyo Women’s medical University

 

IMMUNOHISTOCHEMISTRY
Procedure
1)

Paraffin sections were deparaffinized and rehydrated.

2)

Sections were quenched for several minutes with 3% hydrogen peroxide, rinsed in PBS, pretreated for 30 min with 3% nonimmune animal serum in PBS.

3)

Sections were incubated overnight at 4°C with a antibody at a dilution of 1 micro gram/mL.

4)

Antibody binding was visualized by the avidin-biotin-immuno peroxidase complex method using the Vectastain ABC kit (Vector, Burlingame, CA) according to the manufacturer's instructions.

5)

3,3-Diaminobenzidine tetrahydrochloride was used as the chromogen.

6)

Hematoxylin or Eosin was used as the counter stain.

 

Technical Note
1)

Sections from which the primary antibodies were omitted served
as negative reaction controls.

2)

For frozen materials, samples were fixed in 10% formalin, immersed
in 30% sucrose in PBS, embedded at OCT (Sakura, Tokyo, Japan),
and stored at 80°C.

3)

For paraffin embedded materials, samples were fixed in 10% formalin, dehydrated, embedded in paraffin, and stored at room temperature.

4)

Microwave treatment improves the target staining, whereas it declines
non-specific background.

5)

Protease K treatment declines the target staining.

Positive control
Aortic wall in the renal tissue of diabetic nephropathy or aortic wall of atherosclerosis.

REFERENCES

1)

Protein-bound acrolein: Potential markers for oxidativestress. K.Uchida, M.Kanematsu, K.Sakai, T.Matsuda, N.Hattori,Y.Mizuno, D.Suzuki,T.Miyata, N.Noguchi, E.Niki,T.Osawa
Proc.Natl.Acad.Sci.USA,95,4882-4887(1998)

2)

Protein-bound acrolein: A novel markers of oxidativestress in
Alzheimer's Disease. Noel Y. Calingasan, Koji Uchida,
and Gary E.Gibson
Journal of Neurochemistry.72(2),751-756(1999)

3)

A 1-Hour Enzyme-Linked Immunosorbent Assay for Quantitation
of Acrolein- and Hydroxynonenal- Modified Proteins by Epitope-Bound
CaseinMatrix Method. K.Satoh,S.Yamada,Y.Koike,Y.Igarashi,S.Toyokuni,T.Kumano,T.Takahata,
S.Tsuchida and K.Uchida
Analytical Biochemistry.270,323-328(1999)

4)

Red blood cells inhibit activation-induced cell death and oxidative
stress in human peripheral blood T lymphocytes. Fonseca AM, Porto G,
Uchida K, Arosa FA.
Blood. 97(10), p3152-60 (2001)

5)

Thiolation of protein-bound carcinogenic aldehyde.
An electrophilic acrolein-lysine adduct that covalently binds to thiols.
Furuhata A, Nakamura M, Osawa T, Uchida K.
J Biol Chem. 277(31), p27919-27926 (2002)

The ‘Anti-Acrolein Monoclonal Antibody’ is manufactured in Japan
by the Life Science Products Division of the Nippon Oils and Fats
Corporation, Japan. Genox is the distributor of this product
for users in North and South America.

ORDER THIS PRODUCT

Note: Genox’s Products are for RESEARCH USE ONLY.
Users of Genox’s products are strongly advised NOT TO USE THEM FOR CLINICAL/DIAGNOSTIC APPLICATIONS. 

The provision of test reports, generated by using Genox's products, to individuals
or to treating physicians for the diagnosis, prevention, treatment and control of any human disease or impairment of, or the assessment of the health, nutritional, or medical condition of individuals is expressly prohibited by law.
  42 U.S.C. § 263a (2006); 42 C.F.R. § 493.3 (2006); COMAR 10.10.01.02 (2006). Genox is not a CLIA certified laboratory.

© Copyright 1996-2016 Genox Corporation. All rights reserved.

ELISA Testing Kits
Genox Corporation has
developed biomarker
assays to measure
several oxidative
damage biomarkers.
upcoming events