Genox

Anti-Malondialdehyde Monoclonal Antibody
(Anti Malondialdehyde monoclonal antibody, clone #1F83)
(Catalog #MMDA-030 for 30 microgram vial)

Product Insert (pdf)pdf

What is Malondialdehyde?
Malondialdehyde (MDA) is one of the major aldehydes derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.

mono2a

 

Clone #

:

1F83

Antigen

:

MDA-modified keyhole-lympet hemocyanine.

Form

:

Frozen (100 micro g/mL antibody in 10mM PBS containing 0.1 %NaN3 and 0.5% BSA). Purified by Protein-A.

Application

:

Immunohistochemistry.
Recommended antibody concentration is 0.5-1.0 micro g/mL on paraformaldehyde fixed tissue.

Specificity

:

Specific for MDA-modified protein (especially DHP-lysine).

Subclass

:

Mouse IgG2a(lambda)

Storage

:

Less than -20°C

Specificity of anti-MDA antibody.

 


Immunohistochemical detection of MDA-modified protein in atherosclerotic aorta.
Dr. N Shibata, Tokyo Women's Medical University.

 

IMMUNOHISTOCHEMISTRY
Procedure
1)

Paraffin sections were deparaffinized and rehydrated.

2)

Sections were quenched for several minutes with 3% hydrogen peroxide, rinsed in PBS, pretreated for 30 min with 3% nonimmune animal serum in PBS.

3)

Antigen retrieval by microwave treatment.

4)

Sections were incubated overnight at 4°C with a antibody at a concentration of 0.5-10 micro gram/mL.

5)

Antibody binding was visualized by the avidin-biotin-immuno peroxidase complex method using the Vectastain ABC kit (Vector, Burlingame, CA) according to the manufacturer's instructions.

6)

3,3-Diaminobenzidine tetrahydrochloride was used as the chromogen.

7)

Hematoxylin or Eosin was used as the counter stain.

 

 

REFERENCES

1)

S Yamada, et. al.:  Immunochemical detection of a lipofuscin-like fluophore derived from malondialdehyde and lysine.
Journal of Lipid Research Vol.42, p1187-1196 (2001)
This paper is a good example for immune-histochemical detection of MDA-modified protein and western blot analysis of oxidized LDL.

The ‘Anti-MDA Monoclonal Antibody’ is manufactured in Japan
by the Life Science Products Division of the Nippon Oils and Fats
Corporation, Japan. Genox is the distributor of this product
for users in North and South America.

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Note: Genox’s Products are for RESEARCH USE ONLY.
Users of Genox’s products are strongly advised NOT TO USE THEM FOR CLINICAL/DIAGNOSTIC APPLICATIONS. 

The provision of test reports, generated by using Genox's products, to individuals
or to treating physicians for the diagnosis, prevention, treatment and control of any human disease or impairment of, or the assessment of the health, nutritional, or medical condition of individuals is expressly prohibited by law.
  42 U.S.C. § 263a (2006); 42 C.F.R. § 493.3 (2006); COMAR 10.10.01.02 (2006). Genox is not a CLIA certified laboratory.

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