What is ORAC Assay?

The Oxygen Radical Absorption Capacity (ORAC) assay is a method for quantitatively measuring total antioxidant activity of the sample to be tested.  The assay utilizes the free radical sensitive fluorescent indicator protein beta-phycoerythrin (β-PE) to monitor the effectiveness of various biological antioxidants in protecting the β-PE from becoming oxidized by free radicals.

Assay results are configured by allowing the reaction to reach completion and then integrating the area under the kinetic curve relative to a blank reaction containing no antioxidant protection. The area under the curve is proportional to the concentration of all the antioxidants present in the sample. Example of a typical serum whole ORAC Assay Curve.

Each reaction is calibrated using known standards of Trolox®, a water soluble vitamin E analogue. The results of the assay are reported on the basis of 1 ORAC unit = 1 micro-M Trolox®.

Note: Genox’s Products are for RESEARCH USE ONLY.
Users of Genox’s products are strongly advised NOT TO USE THEM FOR CLINICAL/DIAGNOSTIC APPLICATIONS.

The provision of test reports, generated by using Genox's products, to individuals
or to treating physicians for the diagnosis, prevention, treatment and control of any human disease or impairment of, or the assessment of the health, nutritional, or medical condition of individuals is expressly prohibited by law.
  42 U.S.C. § 263a (2006); 42 C.F.R. § 493.3 (2006); COMAR (2006). Genox is not a CLIA certified laboratory.

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Genox Corporation has
developed biomarker
assays to measure
several oxidative
damage biomarkers.
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