Japan Institute for the Control of Aging: JaICA Oxidative Stress
Anti dityrosine (DT) monoclonal
A novel biomarker for protein oxidation:
Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation.
Detection of DT in protein samples:

DT staining of mouse skin.
(Dr. Sugiyama, Tottori Univ.)
Anti dityrosine monoclonal antibody clone 1C3 has been established by Dr. Toshihiko Osawa (Nagoya Univ.) and Dr. Yoji Kato (Univ. Hyogo)1). They have prepared this antibody using a dimer of 3-(p-hydroxyphenyl) propionic acid as a hapten, and screened using dityrosine-conjugated BSA. This antibody is highly specific for dityrosine, and can react with both free dityrosine and dityrosine containing protein or peptides. Suitable for immunohistochemistry, western blotting and ELISA.
Antigen: 3-(p-hydroxyphenyl) propionic acid dimer-conjugated keyhole limpet hemocyanin.
Form: Lyophilized power. Reconstitute by 200 µL of distilled water. 100 µg/mL of IgG.
Subclass: Mouse IgG2a(kappa), clone 1C3. Protein A purified.
Specificity: Specific for free dityrosine, 3-(p-hydroxyphenyl) propionic acid dimer, dityrosine-BSA conjugate, and dityrosine in protein or peptides. Cross reactivity have been tested for following analogues: Nitrotyrosine, chlorotyrosine, aminotyrosine, phosphotyrosine, tyrosine, 3-(p-hydroxyphenyl) propionic acid, phenylalanine, 3,4-dihydroxyphenylacetic acid (DOPA)
Application: Immunohistochemistry, western blotting and ELISA
Storage: Less than -20°C
1) Kato Y, Wu X, Naito M, Nomura H, Kitamoto N, Osawa T. Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody. Biochem Biophys Res Commun. 275(1), p11-15 (2000).
Product name Code Content
Anti dityrosine (DT) monoclonal antibody MDT-020P 20 µg IgG
FOR RESEARCH USE ONLY. Not for diagnostic, medical or other use.