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Oxidative Stress
Rev.150727 |
New 8-OHdG Check ELISA
ELISA kit for 8-hydroxy-2'-deoxyguanosine |
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| About 8-hydroxy-2'-deoxyguanosine (8-OHdG): |
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8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical,
singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials.
New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is
highly specific for DNA damage, not cross react with RNA oxidation
products such as 8-hydroxy-guanine and 8-hydroxy-guanosine.
This product is suitable for detection of 8-OHdG in urine and other biomaterials from human and animals.
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This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1)
which is highly specific for 8-OHdG.
We provide two types of 8-OHdG ELISA kits with different assay range. New 8-OHdG Check ELISA is suitable for urine and serum
sample from animal and human. If you are planning to measure 8-OHdG in human serum,tissue, cultured cells,
we recommend to use 'Highly Sensitive 8-OHdG Check ELISA'.
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| Specifications |
| Assay principle: |
Competitive ELISA (detection: 450 nm) |
| Specifity: |
Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G,
6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea,
creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
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| Measuring range: |
0.5 to 200 ng/mL |
| Format: |
96 wells (18 samples in triple assays) |
| Applications: |
Urine from human and animals.
For serum and tissue samples,
Highly Sensitive 8-OHdG Check (code #KOG-HS10E) is recommended. |
| Storage: |
Store at 4 - 10°C (don't freeze). |
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| Product name |
Code |
Assay range |
Format |
Application |
| New 8-OHdG Check |
KOG-200SE |
0.5-200 ng/mL |
96 wells |
Urine and animal serum. |
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| FOR RESEARCH USE ONLY. Not for diagnostic, medical or other use. |
| Content of this kit |
| 8-OHdG Microtiter Plate: |
Precoated with 8-OHdG(12 X 8wells, split type) |
1 plate |
| Primary Antibody: |
Anti 8-OHdG antibody, powder. |
1 vial |
| Primary Antibody Solution |
|
1 vial (6mL) |
| Secondary Antibody: |
HRP-anti mouse antibody, powder. |
1 vial |
| Secondary Antibody Solution: |
|
1 vial (12mL) |
| Chromatic Solution: |
3,3',5,5'-tetramethylbenzidine |
1 vial (0.25mL) |
| Diluting Solution: |
H2O2 containing buffer. |
1 vial (12mL) |
| Washing Solution(5x): |
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2 vials (26mL x 2) |
| Reaction Terminating Solution: |
1M Phosphoric acid. |
1 vial (12mL) |
| Standard 8-OHdG Solution: |
Purified 8-OHdG (0.5, 2, 8, 20, 80, 200 ng/mL) |
1 vial each |
| Plate Seal: |
|
2 sheets |
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| References |
| 1) |
H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular
DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986)
[ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ] |
| 2) |
R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991)
[ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ] |
| 3) |
S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa:
Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1:
Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997)
[ Specifity of the antibody clone N45.1.] |
| 4) |
Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K:
Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA.
Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000)
[ Development and assessment of 8-OHdG Check ELISA. ] |
| 5) |
Nagayoshi Y, Kawano H, Hokamaki J, Miyamoto S, Kojima S, Shimomura H, Tsujita K, Sakamoto T,
Yoshimura M, Ogawa H:
Urinary 8-hydroxy-2'-deoxyguanosine levels increase after reperfusion in acute myocardial infarction and may predict subsequent
cardiac events.
Am J Cardiol. 95(4), p514-517 (2005)
[ Measurement of 8-OHdG in human urine. ] |
| 6) |
Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas MJ, Lopez FJ, Abellan P, Villegas JA, Gonzalez-Gallego J:
The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled
double-blind study in cyclists.
Eur J Appl Physiol 95(5-6),p543-549(2005)
[ Study about exercise, antioxidant supplements and urinary 8-OHdG. ] |
| 7) |
Someya T, Kaneko K, Yamada T, Yamashiro Y:
Effect of a novel free radical scavenger, edaravone, on puromycin aminonucleoside induced nephrosis in rats.
Pediatr Nephrol 20(10),p1430-1434(2005)
[ Effect of antioxidants to 8-OHdG in rat urine. ] |
| 8) |
Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K:
Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated
with a peripheral marker of oxidative DNA damage.
J Neurol Sci 250(1-2),p159-161(2006)
[ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ] |
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| FOR RESEARCH USE ONLY. Not for diagnostic, medical or other use. |
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