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| Suitable for assessment of oxidative stress in serum, tissue and cultured cells. For research use only. |
| About 8-hydroxy-2'-deoxyguanosine (8-OHdG): |
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8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical,
singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials.
New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is
highly specific for DNA damage, not cross react with RNA oxidation
products such as 8-hydroxy-guanine and 8-hydroxy-guanosine.
This product is suitable for detection of 8-OHdG in urine and other biomaterialsfrom human and animals.
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This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1)
which is highly specific for 8-OHdG.
We provide two types of 8-OHdG ELISA kits with different assay range. Highly Sensitive 8-OHdG Check ELISA is suitable for urine, serum
, tissue and cultured cells. |
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| Specifications |
| Assay principle: |
Competitive ELISA (detection: 450 nm) |
| Specifity: |
Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G,
6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea,
creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
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| Measuring range: |
0.125 to 10 ng/mL |
| Format: |
96 wells (18 samples in triple assays) |
| Applications: |
Urine, serum, tissue and cultured cells. |
| Storage: |
Store at 4 - 10°C (don't freeze). |
| Expiry: |
9 months after the day of manufacturing. |
| Required but not provided: |
Micropipet and chip (100 µL, 1000 µL).
Measuring pipet (10 mL, 20 mL)/ measuring cylinders.
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
Microplate reader (filter; 450 nm). |
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| Content of this kit |
| 8-OHdG Microtiter Plate: |
Precoated with 8-OHdG(12 X 8wells, split type) |
1 plate |
| Primary Antibody: |
Anti 8-OHdG antibody, powder. |
1 vial |
| Primary Antibody Solution |
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1 vial (6mL) |
| Secondary Antibody: |
HRP-anti mouse antibody, powder. |
1 vial |
| Secondary Antibody Solution: |
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1 vial (12mL) |
| Chromatic Solution: |
3,3',5,5'-tetramethylbenzidine |
1 vial (0.25mL) |
| Diluting Solution: |
H2O2 containing buffer. |
1 vial (12mL) |
| Washing Solution(5x): |
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2 vials (26mLx2) |
| Reaction Terminating Solution: |
1M Phosphoric acid. |
1 vial (12mL) |
| Standard 8-OHdG Solution: |
Purified 8-OHdG (0.125, 0.25, 0.5, 1, 4, 10 ng/mL) |
1 vial each |
| Plate Seal: |
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2 sheets |
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| References |
| 1) |
H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular
DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986)
[ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ] |
| 2) |
R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991)
[ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ] |
| 3) |
S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa:
Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1:
Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997)
[ Specifity of the antibody clone N45.1.] |
| 4) |
Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K:
Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA.
Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000)
[ Development and assessment of 8-OHdG Check ELISA. ] |
| 5) |
M.D.Evans, M.S.Cooke, I.D.Podmore, Q.Zheng, K.E.Herbert and J.Lunec:
Discrepancies in the measurement of UVC-induced 8-oxo-2'-deoxyguanosine: Implications for the analysis of oxidative DNA damage.
Biochem Biophys Res Commun 259, p374-378 (1999)
[ ELISA results show high correlation between those of HPLC-ECD when 8-OHdG in DNA samples are assessed. ] |
| 6) |
Ha Won Kim, Akira Murakami, Marshall V Williams and Hajime Ohigashi:
Mutagenicity of reactive oxygen and nitrogen species as detected by co-culture of activated inflammatory leukocytes and AS52 cells.
Carcinogenesis 24(2), p235-241 (2003)
[ Detection of 8-OHdG in cultured cells. ] |
| 7) |
Watanabe E, Matsuda N, Shiga T, Kajimoto K, Ajiro Y, Kawarai H, Kasanuki H, Kawana M:
Significance of 8-hydroxy-2'-deoxyguanosine levels in patients with idiopathic dilated cardiomyopathy.
J Card Fail. 2006 Sep;12(7):527-32.
[ Serum 8-OHdG in IDC patients are assessed. ] |
| 8) |
Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K:
Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated
with a peripheral marker of oxidative DNA damage.
J Neurol Sci. 250(1-2):159-61(2006)
[ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ] |
| 9) |
Reiko Nagasaka, Nobuaki Okamoto, Hideki Ushio:
Effects of caloric restriction on post-spawning death of ayu.
Exp Gerontol 40, p556-561(2005)
[ Detection of 8-OHdG in fish tissues (brain and liver). ] |
| 10) |
Yasuda M, Ide H, Furuya K, Yoshii T, Nishio K, Saito K, Isotani S, Kamiyama Y, Muto S, Horie S:
Salivary 8-OHdG: a useful biomarker for predicting severe ED and hypogonadism.
J Sex Med. 5(6),p1482-1491(2008)
[ 8-OHdG in saliva can be detected by 8-OHdG ELISA. ] |
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| Product name |
Code |
Assay range |
Application |
| Highly Sensitive 8-OHdG Check |
KOG-HS10E |
0.125 - 10 ng/mL |
Serum, plasma, tissue and other biological fluids. |
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Made in Japan. |
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| Contact technical support |
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