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| Suitable for assessment of oxidative stress using urine samples. For research use only. |
| About 8-hydroxy-2'-deoxyguanosine (8-OHdG): |
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8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical,
singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials.
New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is
highly specific for DNA damage, not cross react with RNA oxidation
products such as 8-hydroxy-guanine and 8-hydroxy-guanosine.
This product is suitable for detection of 8-OHdG in urine and other biomaterials from human and animals.
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This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1)
which is highly specific for 8-OHdG.
We provide two types of 8-OHdG ELISA kits with different assay range. New 8-OHdG Check ELISA is suitable for urine and serum
sample from animal and human. If you are planning to measure 8-OHdG in human serum,tissue, cultured cells,
we recommend to use ' Highly Sensitive 8-OHdG Check ELISA'. |
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| Specifications |
| Assay principle: |
Competitive ELISA (detection: 450 nm) |
| Specifity: |
Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G,
6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea,
creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
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| Measuring range: |
0.5 to 200 ng/mL |
| Format: |
96 wells (18 samples in triple assays) |
| Applications: |
Urine from human and animals.
For serum and tissue samples,
Highly Sensitive 8-OHdG Check (code #KOG-HS10E) is recommended. |
| Storage: |
Store at 4 - 10°C (don't freeze). |
| Expiry: |
12 months after the day of manufacturing. |
| Required but not provided: |
Micropipet and chip (100 micro L, 1000 micro L).
Measuring pipet (10 mL, 20 mL)/ measuring cylinders.
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
Microplate reader (filter; 450 nm). |
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| Content of this kit |
| 8-OHdG Microtiter Plate: |
Precoated with 8-OHdG(12 X 8wells, split type) |
1 plate |
| Primary Antibody: |
Anti 8-OHdG antibody, powder. |
1 vial |
| Primary Antibody Solution |
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1 vial (6mL) |
| Secondary Antibody: |
HRP-anti mouse antibody, powder. |
1 vial |
| Secondary Antibody Solution: |
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1 vial (12mL) |
| Chromatic Solution: |
3,3',5,5'-tetramethylbenzidine |
1 vial (0.25mL) |
| Diluting Solution: |
H2O2 containing buffer. |
1 vial (12mL) |
| Washing Solution(5x): |
|
2 vials (26mL x 2) |
| Reaction Terminating Solution: |
1M Phosphoric acid. |
1 vial (12mL) |
| Standard 8-OHdG Solution: |
Purified 8-OHdG (0.5, 2, 8, 20, 80, 200 ng/mL) |
1 vial each |
| Plate Seal: |
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2 sheets |
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| References |
| 1) |
H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular
DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986)
[ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ] |
| 2) |
R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991)
[ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ] |
| 3) |
S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa:
Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1:
Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997)
[ Specifity of the antibody clone N45.1.] |
| 4) |
Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K:
Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA.
Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000)
[ Development and assessment of 8-OHdG Check ELISA. ] |
| 5) |
Nagayoshi Y, Kawano H, Hokamaki J, Miyamoto S, Kojima S, Shimomura H, Tsujita K, Sakamoto T,
Yoshimura M, Ogawa H:
Urinary 8-hydroxy-2'-deoxyguanosine levels increase after reperfusion in acute myocardial infarction and may predict subsequent
cardiac events.
Am J Cardiol. 95(4), p514-517 (2005)
[ Measurement of 8-OHdG in human urine. ] |
| 6) |
Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas MJ, Lopez FJ, Abellan P, Villegas JA, Gonzalez-Gallego J:
The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled
double-blind study in cyclists.
Eur J Appl Physiol 95(5-6),p543-549(2005)
[ Study about exercise, antioxidant supplements and urinary 8-OHdG. ] |
| 7) |
Someya T, Kaneko K, Yamada T, Yamashiro Y:
Effect of a novel free radical scavenger, edaravone, on puromycin aminonucleoside induced nephrosis in rats.
Pediatr Nephrol 20(10),p1430-1434(2005)
[ Effect of antioxidants to 8-OHdG in rat urine. ] |
| 8) |
Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K:
Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated
with a peripheral marker of oxidative DNA damage.
J Neurol Sci 250(1-2),p159-161(2006)
[ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ] |
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| Product name |
Code |
Assay range |
Format |
Application |
| New 8-OHdG Check |
KOG-200SE |
0.5-200 ng/mL |
96 wells |
Urine and animal serum. |
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Made in Japan. |
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| Contact technical support |
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[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would causedby such faults, we will be exempt from responsibility.
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Genox Corporation. All rights reserved.
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