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Biomarkers for lipid oxidation. oxidative stress
Anti methylglyoxal/ argpyrimidine monoclonal antibody.
Suitable for immunohistocheistry. For research use only.
About methylglyoxal (MG)
Methylglyoxal (MG), an endogenous metabolite that increases in diabetes and is a common intermediate in the Maillard reaction (glycation), reacts with proteins and forms advanced glycation end products (AGE). MG reacts with arginine residue in protein and forms numerous numbers of adducts, such as argpyrimidine. This antibody is specific for argpyrimidine: N-(5-hydroxy-4,6-dimethylpyrimidine-2-yl)-L-ornithine.
Specifications
Clone #: 3C
Antigen: MG-modified keyhole-lympet hemocyanine
Specificity: Specific for MG-modified protein (especially Argpyrimidine)
Subclass: Mouse IgG2a
Form: Frozen (10mM PBS containing 0.1% NaN3 and 0.5% BSA, 100 µg/mL IgG.
Protein A purified.
Application: Immunohistochemistry°®It is recommended that the antibody be tried at 0.5-1.0 µg/mL on paraformaldehyde fixed tissue.
Storage: Less than -20°C
 
Specificity of anti MG antibody. Immunohistochemical detection of MG adducts in atherosclerotic aorta.
Noriyuki Shibata et al. Tokyo Women°«s medical University
References
1) Protein modification by a Maillard reaction intermediate methylglyoxal. Immunochemical detection of fluorescent 5-methylimidazolone derivatives in vivo. Uchida K, Khor OT, Oya T, Osawa T, Yasuda Y, Miyata T. FEBS Lett. 410(2-3), p313-318. (1997)
2) Methylglyoxal Modification of Protein -Chemical and Immunochemical Characterization of Methylglyoxal-Arginine Adduct. T.Oya, N.Hattori, Y.Mizuno, S.Miyata, S.Maeda, T.Osawa and K.Uchida. J.Biological Chemistry, 274, 18492-18502 (1999)
3) Advanced glycation and lipidoxidation of the peritoneal membrane: respective roles of serum and peritoneal fluid reactive carbonyl compounds. Miyata T, Horie K, Ueda Y, Fujita Y, Izuhara Y, Hirano H, Uchida K, Saito A, van Ypersele de Strihou C, Kurokawa K. Kidney Int. 58(1), p425-435.(2000)
4) Argpyrimidine, a blue fluorophore in human lens proteins: high levels in brunescent cataractous lenses. Padayatti PS, Ng AS, Uchida K, Glomb MA, Nagaraj RH. Invest Ophthalmol Vis Sci. 42(6), p1299-1304. (2001)
5) High concentrations of glucose induce synthesis of argpyrimidine in retinal endothelial cells. Padayatti PS, Jiang C, Glomb MA, Uchida K, Nagaraj RH. Curr Eye Res. 23(2), p106-115. (2001)
6) Curcumin Inhibits ROS Formation and Apoptosis in Methylglyoxal- Treated Human Hepatoma G2 Cells. WEN-HSIUNG CHAN, HSIN-JUNG WU and YAN-DER HSUUW. Ann. N.Y. Acad. Sci. 1042: 372-378 (2005)
Product name Code Content
Anti Methylglyoxal (MG) monoclonal antibody MMG-030n 30 µg IgG

Manufactured by NOF corporation, Japan.

Ordering Information
Instruction manual (PDF) Technical information
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.

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