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In this web site, technical information about trouble shootings, application examples and
list of reference papers are presented. If you have any questions or any comments,
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| Type |
Product name / code |
Click below |
DNA
oxidation |
8-OHdG Check ELISA (Code. KOG-200SE / KOG-HS10E) |
Tech Info |
Ref. |
| Anti 8-OHdG antibody (N45.1) (Code: MOG-020P / MOG-100P) |
Tech Info |
Ref. |
| Anti Thymidine Glycol (TG) antibody (Code: MTG-100P) |
Tech Info |
Ref. |
Lipid
oxidation |
Hexanoyl-Lysine (HEL) ELISA kit (Code: KHL-700E) |
Tech Info |
Ref. |
| Anti Hexanoyl-Lysine (HEL) antibody (Code: MHL-021P / MHL-020P) |
Tech Info |
Ref. |
| Anti 4-HNE antibody (HNEJ2) (Code: MHN-020P / MHN-100P) |
Tech Info |
Ref. |
| Anti Acrolein (ACR) antibody (5F6) (Code: MAR-020n / MAR-100n) |
Tech Info |
Ref. |
| Anti MDA antibody (1F83) (Code: MMD-030n) |
Tech Info |
Ref. |
| Anti 4-hydroxy-2-hexenal (4-HHE) antibody (Code: MHH-030n) |
Tech Info |
Ref. |
| Anti Crotonaldehyde (CRA) antibody (Code: MCA-030n) |
Tech Info |
Ref. |
| Anti Methylglyoxal (MG) antibody (Code: MMG-030n) |
Tech Info |
Ref. |
| Anti 7-ketocholesterol (7-KC) antibody (Code: MKC-020n) |
Tech Info |
Ref. |
Protein
oxidation |
Dityrosine (DT) ELISA (Code: KDT-010E) |
Tech Info |
Ref. |
| Anti Dityrosine (DT) antibody (Code: MDT-020P) |
Tech Info |
Ref. |
| Anti Dibromo-Tyrosine (DiBrY) antibody (Code: MBY-020P) |
Tech Info |
Ref. |
Antioxidant
assay |
Test kit for Potential Antioxidant(PAO) (Code: KPA-050) |
Tech Info |
Ref. |
| Traceelements Assay |
Fe / Iron Assay Kit (ferrozine) (Code: CFE-005) |
Tech Info |
Ref. |
| Fe / Iron Assay Kit (Nitroso-PSAP) (Code: CFE-010) |
Tech Info |
Ref. |
| UIBC Assay Kit (Bathophenanthroline) (Code: CBC-800) |
Tech Info |
Ref. |
| Cu / Copper Assay Kit (3,5-DiBr-PAESA) (Code: CCU-400) |
Tech Info |
Ref. |
| Zn / Zinc Assay Kit (5-Br-PAPS) (Code: CZN-001) |
Tech Info |
Ref. |
| Ca / Calsium Assay Kit (Chlorophosphonazo III) (Code: CCA-020) |
Tech Info |
Ref. |
| Ca / Calsium Assay Kit (o-Cresolphtalein-Complexone) (Code: CCA-030) |
Tech Info |
Ref. |
| Mg / Magnesium Assay Kit (Xyridil Blue-I) (Code: CMG-035) |
Tech Info |
Ref. |
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| Fe / Iron Assay Kit (ferrozine method) |
| No. |
Title |
Content |
| 1 |
Sample storage conditions |
Tissue samples should be stored as tissue block or homogenates frozen at -80°C.
Please avoid preservatives, anti-freeze agents, formalin, chelating agents such as EDTA, etc.
If possible, remode blood by saline.
Frozen tissue can be stored at least a few months. |
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Ask a question about Fe/Iron Assay Kit by e-mail.
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| List of references: |
| 1) |
Y Ikeda, S Tajima, YI-Ishizawa, Y Kihira, K Ishizawa, S Tomita, K Tsuchiya and T Tamaki:
Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in Hepatocytes.
PLoS ONE 7(7),e40465(2012) doi: 10.1371/journal.pone.0040465
|
| 2) |
Tsugawa H, Suzuki H, Matsuzaki J, Hirata K, Hibi T:
FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach.
Free Radical Biology and Medicine 52(6),p1003-1010(2012) doi: 10.1016/j.freeradbiomed.2011.12.011.
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| 3) |
M Nakaya, M Tajima, H Kosako, T Nakaya, A Hashimoto, K Watari, H Nishihara, M Ohba,
S Komiya, N Tani, M Nishida, H Taniguchi, Y Sato, M Matsumoto, M Tsuda, M Kuroda, K Inoue and H Kurose:
GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance.
Nature Communications 4, 1532 (2013) doi:10.1038/ncomms2540
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| 4) |
Y Ikeda, H Enomoto, S Tajima, YI Ishizawa, Y Kihira, K Ishizawa, S Tomita, K Tsuchiya,
T Tamaki:
Dietary iron restriction inhibits progression of diabetic nephropathy in db/db mice.
American Journal of Physiology - RenalPhysiology 304:F1028-F1036(2013) DOI:10.1152/ajprenal.00473.2012
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| 5) |
Y Ikeda, I Ozono, S Tajima, M Imao, Y Horinouchi, YI Ishizawa, Y Kihira, L Miyamoto,
K Ishizawa, K Tsuchiya, T Tamaki:
Iron Chelation by Deferoxamine Prevents Renal Interstitial Fibrosis in Mice with Unilateral Ureteral Obstruction.
PLoS ONE 9(2), e89355(2014) doi:10.1371/journal.pone.0089355
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Ask a question about Fe/Iron Assay Kit by e-mail.
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| Fe / Iron Assay Kit (Nitroso-PSAP method: Code. CFE-010) |
| No. |
Title |
Content |
| 1 |
Application to tissue samples |
CFE-010 can be applied to cell lysates and tissue homogenates.
Add 5% TCA solution. Please confirm that pH of the mixture is pH2 - 3.
Incubate for 30 minutes at 4-8 °C.
Centrifuge for 15 minutes at 6,000 rpm.
Collect the supernatant, and apply to CFE-010.
NOTE: Centrifuge should be performed after acid extraction. |
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Ask a question about Fe/Iron Assay Kit (Nitroso-PSAP) by e-mail.
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| List of references: |
| 1) |
Tsugawa H, Suzuki H, Matsuzaki J, Hirata K, Hibi T:
FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach.
Free Radic Biol Med 52(6),p1003-1010(2012).
DOI: 10.1016/j.freeradbiomed.2011.12.011
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| 2) |
Yasumasa Ikeda, Soichiro Tajima, Yuki Izawa-Ishizawa, Yoshitaka Kihira, Keisuke Ishizawa,
Shuhei Tomita, Koichiro Tsuchiya, and Toshiaki Tamaki:
Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in Hepatocytes.
PLoS One. 2012; 7(7): e40465.
doi: 10.1371/journal.pone.0040465
|
| 3) |
Nakaya M, Tajima M, Kosako H, Nakaya T, Hashimoto A, Watari K, Nishihara H, Ohba M,
Komiya S, Tani N, Nishida M, Taniguchi H, Sato Y, Matsumoto M, Tsuda M, Kuroda M, Inoue K, Kurose H:
GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance.
Nat Commun 4:p1532(2013).
doi: 10.1038/ncomms2540. |
| 4) |
Ikeda Y, Enomoto H, Tajima S, Izawa-Ishizawa Y, Kihira Y, Ishizawa K, Tomita S, Tsuchiya K,
Tamaki T:
Dietary iron restriction inhibits progression of diabetic nephropathy in db/db mice.
Am J Physiol Renal Physiol 304(7):F1028-36(2013)
doi: 10.1152/ajprenal.00473.2012. |
| 5) |
Hayashi K, Nakamura M, Sakamoto W, Yogo T, Miki H, Ozaki S, Abe M, Matsumoto T, Ishimura K:
Superparamagnetic nanoparticle clusters for cancer theranostics combining magnetic resonance imaging and hyperthermia treatment.
Theranostics 3(6),p366-376(2013)
doi: 10.7150/thno.5860. |
| 6) |
Isobe N, Chen X, Kim UJ, Kimura S, Wada M, Saito T, Isogai A:
TEMPO-oxidized cellulose hydrogel as a high-capacity and reusable heavy metal ion adsorbent.
J Hazard Mater 260,p195-201(2013)
doi: 10.1016/j.jhazmat.2013.05.024. |
| 7) |
Ikeda Y, Ozono I, Tajima S, Imao M, Horinouchi Y, Izawa-Ishizawa Y, Kihira Y, Miyamoto L,
Ishizawa K, Tsuchiya K, Tamaki T:
Iron chelation by deferoxamine prevents renal interstitial fibrosis in mice with unilateral ureteral obstruction.
PLoS One 9(2):e89355(2014)
doi: 10.1371/journal.pone.0089355. |
| 8) |
Kagoya Y, Yoshimi A, Tsuruta-Kishino T, Arai S, Satoh T, Akira S, Kurokawa M:
JAK2V617F+ myeloproliferative neoplasm clones evoke paracrine DNA damage to adjacent normal cells through secretion of
lipocalin-2.
Blood 124(19),p2996-3006(2014)
doi: 10.1182/blood-2014-04-570572. |
| 9) |
Imamura T, Hirayama T, Tsuruma K, Shimazawa M, Nagasawa H, Hara H:
Hydroxyl radicals cause fluctuation in intracellular ferrous ion levels upon light exposure during photoreceptor cell death.
Exp Eye Res 129,p24-30(2014)
doi: 10.1016/j.exer.2014.10.019. |
| 10) |
Tajima S, Ikeda Y, Enomoto H, Imao M, Horinouchi Y, Izawa-Ishizawa Y, Kihira Y, Miyamoto L,
Ishizawa K, Tsuchiya K, Tamaki T:
Angiotensin II alters the expression of duodenal iron transporters, hepatic hepcidin, and body iron distribution in mice.
Eur J Nutr 54(5),p709-719(2015)
doi: 10.1007/s00394-014-0749-1. |
| 11) |
Eguchi A, Naito Y, Iwasaku T, Okuhara Y, Morisawa D, Sawada H, Nishimura K, Oboshi M, Fujii K,
Mano T, Masuyama T, Hirotani S:
Association of dietary iron restriction with left ventricular remodeling after myocardial infarction in mice.
Heart Vessels 31(2),p222-229(2016)
doi: 10.1007/s00380-014-0621-5. |
| 12) |
Ohtsuka H, Ishida M, Naito C, Murakami H, Aiba H.
Sexual development of Schizosaccharomyces pombe is induced by zinc or iron limitation through Ecl1 family genes.
Mol Genet Genomics 290(1),p173-185(2015)
doi: 10.1007/s00438-014-0911-8. |
| 13) |
Nomata J, Maeda M, Isu A, Inoue K, Hisabori T:
Involvement of thioredoxin on the scaffold activity of NifU in heterocyst cells of the diazotrophic cyanobacterium
Anabaena sp. strain PCC 7120.
J Biochem 158(3),p253-261(2015)
doi: 10.1093/jb/mvv046. |
| 14) |
Oboshi M, Naito Y, Sawada H, Hirotani S, Iwasaku T, Okuhara Y, Morisawa D, Eguchi A, Nishimura K,
Fujii K, Mano T, Ishihara M, Masuyama T:
Temporary dietary iron restriction affects the process of thrombus resolution in a rat model of deep vein thrombosis.
PLoS One 10(5):e0126611(2015)
doi: 10.1371/journal.pone.0126611. |
| 15) |
Naito Y, Fujii A, Sawada H, Oboshi M, Iwasaku T, Okuhara Y, Morisawa D, Eguchi A, Hirotani S,
Masuyama T:
Association between renal iron accumulation and renal interstitial fibrosis in a rat model of chronic kidney disease.
Hypertens Res 38(7),p463-470(2015)
doi: 10.1038/hr.2015.14. |
| 16) |
Watanabe S, Sueda R, Fukumori F, Watanabe Y:
Characterization of Flavin-Containing Opine Dehydrogenase from Bacteria.
PLoS One 10(9):e0138434(2015)
doi: 10.1371/journal.pone.0138434. |
| 17) |
Ikeda Y, Imao M, Satoh A, Watanabe H, Hamano H, Horinouchi Y, Izawa-Ishizawa Y, Kihira Y,
Miyamoto L, Ishizawa K, Tsuchiya K, Tamaki T:
Iron-induced skeletal muscle atrophy involves an Akt-forkhead box O3-E3 ubiquitin ligase-dependent pathway.
J Trace Elem Med Biol 35,p66-76(2016)
doi: 10.1016/j.jtemb.2016.01.011. |
| 18) |
Murotomi K, Arai S, Uchida S, Endo S, Mitsuzumi H, Tabei Y, Yoshida Y, Nakajima Y:
Involvement of splenic iron accumulation in the development of nonalcoholic steatohepatitis in Tsumura Suzuki Obese
Diabetes mice.
Sci Rep 6:22476(2016)
doi: 10.1038/srep22476. |
| 19) |
Watanabe S, Tajima K, Fujii S, Fukumori F, Hara R, Fukuda R, Miyazaki M, Kino K, Watanabe Y:
Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase.
Sci Rep 6:38720(2016)
doi: 10.1038/srep38720. |
| 20) |
Tsujino S, Uematsu C, Dohra H, Fujiwara T:
Pyruvic oxime dioxygenase from heterotrophic nitrifier Alcaligenes faecalis is a nonheme Fe(II)-dependent enzyme
homologous to class II aldolase.
Sci Rep 7:39991(2017)
doi: 10.1038/srep39991. |
| 21) |
Yoshida K, Hisabori T:
Distinct electron transfer from ferredoxin-thioredoxin reductase to multiple thioredoxin isoforms in chloroplasts.
Biochem J 474(8),p1347-1360(2017)
doi: 10.1042/BCJ20161089. |
| 22) |
Oshima K, Ikeda Y, Horinouchi Y, Watanabe H, Hamano H, Kihira Y, Kishi S, Izawa-Ishizawa Y,
Miyamoto L, Hirayama T, Nagasawa H, Ishizawa K, Tsuchiya K, Tamaki T:
Iron suppresses erythropoietin expression via oxidative stress-dependent hypoxia-inducible factor-2 alpha inactivation.
Lab Invest 97(5),p555-566(2017)
doi: 10.1038/labinvest.2017.11. |
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Ask a question about Fe/Iron Assay Kit (Nitroso-PSAP) by e-mail.
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| CCU-400: Cu / Copper Assay Kit (3,5-DiBr-PAESA) |
| No. |
Title |
Content |
| 1 |
Application to cultured cells |
CCU-400 can be applied to cultured media and cell homogenates.
Please take care that chelating agents such as EDTA may interfere this assay.
[Assay example]
1) Add HCl at a final concentration of 0.1 M, and extract for 15min - overnight.
2) Centrifuge at 5,000 - 10,000 rpm for 15 min 4 °C
3) Collect the supernatant and apply to CCU-400 assay. |
| 2 |
Aged frozen tissue |
Q: We have frozen tissue which has been frozen in liquid nitrogen
for over 6 months.
Can we use this kind of sample?
A: Yes, but please avoid freeze / thaw cycles.
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Ask a question about Cu / Copper Assay Kit (3,5-DiBr-PAESA) by e-mail.
|
|
|
| List of references: |
| 1) |
Kazuma Nakanishi, Masahiro Tomita and Katsuya Kato:
Synthesis of amino-functionalized mesoporous silica sheets and their application for metal ion capture.
J Asian Ceramic Societies 3(1), p70-76(2015) doi:10.1016/j.jascer.2014.10.011
|
| 2) |
Nobuhiko Asada, Aoi Kedamori, Yumiko Kusano and Tetsuro Takeuchi:
Pheomelanin Formation and Low Tyrosinase Activity in Fading Body Color Variant BdlR Strain Oryzias latipes.
J Life Sci 8(6),p517-521(2014)
|
| 3) |
Yasuyuki Sakurai, Itsuki Anzai, and Yoshiaki Furukawa:
A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide dismutase.
J Biol Chem 289(29),p20139-20149(2014). doi: 10.1074/jbc.M114.567677
|
| 4) |
Sakamoto, Terui Y, Yamamoto T, Kasahara T, Nakamura M, Tomitori H, Yamamoto K, Ishihama A,
Michael AJ, Igarashi K, Kashiwagi K:
Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR
in the two-component signal transducing systems, and ribosome recycling factor.
Int J Biochem Cell Biol. 44(11),p1877-1886(2012). doi: 10.1016/j.biocel.2012.07.010
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Ask a question about Cu / Copper Assay Kit (3,5-DiBr-PAESA) by e-mail.
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| Zn / Zinc Assay Kit (5-Br-PAPS) |
| No. |
Title |
Content |
| 1 |
Application to plant materials
(Acid extraction) |
Typical protocol for acid extraction:
1) Prepare plant homogenates.
2) Add hydrochloric acid (HCl) or Nitric acid at a final concentration of 0.01 - 0.1 mol/L.
3) Mix vigorously and stand for 1 hour.
4) Centrifuge the mixture at 5,000-10,000 rpm for 10 minutes at 4 degree C to remove insoluble materials.
5) Apply to Zn assay kit.
In the application plant materials, breakdown of cell walls would be needed for extraction process.
Please take care to prepare homogenates into fragments small enough to effective extraction.
Experimental conditions can be modified depending on the nature of samples.
If you are planning to detect organic zinc (organic zinc can't be extracted by acid), or
to apply to chelating agent-rich materials, please contact our technical support.
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Ask a question about Zn / Zinc Assay Kit (5-Br-PAPS) by e-mail.
|
|
|
| List of references: |
| 1) |
Kazuma Nakanishi, Masahiro Tomita, Katsuya Kato:
Synthesis of amino-functionalized mesoporous silica sheets and their application for metal ion capture.
Journal of Asian Ceramic Societies 3(1), p70-76(2015) doi:10.1016/j.jascer.2014.10.011
|
| 2) |
Akira Mochizuki and Hideki Kaneda:
Study on the blood compatibility and biodegradation properties of magnesium alloys.
Materials Science and Engineering: C 47(1), p204-210(2015) doi:10.1016/j.msec.2014.11.041
|
| 3) |
Yasuyuki Sakurai, Itsuki Anzai and Yoshiaki Furukawa:
A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide dismutase.
J. Biol. Chem. published online June 10, 2014 doi: 10.1074/jbc.M114.567677
|
| 4) |
Akira Minami, Hiroshi Matsushita, Yuuki Horii, Daisuke Ieno, Yukino Matsuda,
Masakazu Saito, Hiroaki Kanazawa, Yuriko Ohyama, Akihiko Wakatsuki, Atsushi Takeda, Kazuya I. P. J. Hidari,
Vikineswary Sabaratnam, Takashi Suzuki:
Improvement of Depression-Like Behavior and Memory Impairment with the Ethanol Extract of Pleurotus eryngii
in Ovariectomized Rats.
Biological and Pharmaceutical Bulletin 36(12),p1990-1995(2013) doi: 10.1248/bpb.b13-00657
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| 5) |
Homma K, Fujisawa T, Tsuburaya N, Yamaguchi N, Kadowaki H, Takeda K, Nishitoh H,
Matsuzawa A, Naguro I, Ichijo H:
SOD1 as a molecular switch for initiating the homeostatic ER stress response under zinc deficiency.
Mol Cell 52(1),p75-86(2013) doi: 10.1016/j.molcel.2013.08.038
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| 6) |
Noriyuki Isobe, Xiaoxia Chen, Ung-Jin Kim, Satoshi Kimura, Masahisa Wada,
Tsuguyuki Saito, Akira Isogai:
TEMPO-oxidized cellulose hydrogel as a high-capacity and reusable heavy metal ion adsorbent.
Journal of Hazardous Materials 260, p195-201(2013) doi:10.1016/j.jhazmat.2013.05.024
|
| 7) |
Joshua Chou, Jia Hao, Hirokazu Hatoyama, Besim Ben-Nissan, Bruce Milthorpe,
Makoto Otsuka:
The Therapeutic Effect on Bone Mineral Formation from Biomimetic Zinc Containing Tricalcium Phosphate (ZnTCP)
in Zinc-Deficient Osteoporotic Mice.
PLoS ONE 8(8): e71821. doi:10.1371/journal.pone.0071821
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| 8) |
Fei Zhuang and Nobutaka Hanagata:
Synergic Toxicity of Solid Particles and Released Zinc from Zinc Oxide Nanoparticles to Human Lung Epithelial Cells.
Nano Biomedicine 4(2)p90-112(2012) doi: 10.11344/nano.4.90
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Ask a question about Zn / Zinc Assay Kit (5-Br-PAPS) by e-mail.
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| Ca / Calsium Assay Kit (Chlorophosphonazo III) |
| No. |
Title |
Content |
| 1 |
Application to bone samples: |
1) Wash bone sample with saline, desiccate it and measure weight.
2) Mix 0.1g of homogenated bone sample with 1mL of 0.1 M of HCl, and shake overnight.
3) Centrifuge 10,000 rpm for 10 min at 4°C, and collect supernatant.
4) Apply to Ca assay kit (dilution will be needed).
Experimental conditions such as extraction time, HCl concentration and volume should be optimized depending
on your samples. If HCl with higher concentration will be used, please adjust pH between pH2 and 3 by
NaOH solution.
|
| 2 |
Demineralized bone samples |
1) Acid cleavage by nitric acid and hydrogen peroxide, heat at 200 °C.
2) Dissolve dried samples with 0.1M HNO3.
3) Adjust pH between pH2 and 3 by NaOH solution.
4) Apply to Ca assay kit (dilution will be needed).
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Ask a question about Ca / Calsium Assay Kit (Chlorophosphonazo III) by e-mail.
|
|
|
| List of references: |
| 1) |
J. W. Ferguson, J. J. Richard, J. W. O¡Çlaughlin and C. V. Banks:
Simultaneous Spectrophotometric Determination of Calcium Chelate Color and Magnesium with Chlorophosphonazo-III.
Anal Chem 36, p796(1964)
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| 2) |
Michio Zenki, Tomoko Masutani, and Takashi Yokohama:
Repetitive Determination of Calcium Ion and Regeneration of a Chromogenic Reagent Using Chlorophosphonazo III
and anIon Exchanger in a Circulatory Flow Injection System.
Analytical Sciences 18, p1137-1140(2002)
|
| 3) |
Fujita T, Noguchi K, Terashima I:
Apoplastic mesophyll signals induce rapid stomatal responses to CO2 in Commelina communis.
New Phytol 199(2),p395-406(2013)
https://dx.doi.org/10.1111/nph.12261
|
| 4) |
Kenta Maruyama, Satoshi Uematsu, Takeshi Kondo, Osamu Takeuchi, Mikael M. Martino, Takumi Kawasaki,
and Shizuo Akira:
Strawberry notch homologue 2 regulates osteoclast fusion by enhancing the expression of DC-STAMP.
J Exp Med 210(10),p1947-1960(2013).
https://dx.doi.org/10.1084%2Fjem.2013051
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| 5) |
K. Hisano, O. Fujise, M. Miura, T. Hamachi, E. Matsuzaki, F. Nishimura:
The pga gene cluster in Aggregatibacter actinomycetemcomitans is necessary for the development of natural competence
in Ca2+-promoted biofilms.
Molecular Oral Microbiology 29(2),p79-89(2014)
DOI: 10.1111/omi.12046
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Ask a question about Ca / Calsium Assay Kit (Chlorophosphonazo III) by e-mail.
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| Ca / Calsium Assay Kit (o-Cresolphtalein-Complexone: OCPC) CCA-030 |
| No. |
Title |
Content |
| 1 |
Application to bone samples: |
1) Wash bone sample with saline, desiccate it and measure weight.
2) Mix 0.1g of homogenated bone sample with 1mL of 0.1 M of HCl, and shake overnight.
3) Centrifuge 10,000 rpm for 10 min at 4°C, and collect supernatant.
4) Apply to Ca assay kit (dilution will be needed).
Experimental conditions such as extraction time, HCl concentration and volume should be optimized depending
on your samples. If HCl with higher concentration will be used, please adjust pH between pH2 and 3 by
NaOH solution.
|
| 2 |
Demineralized bone samples |
1) Acid cleavage by nitric acid and hydrogen peroxide, heat at 200 °C.
2) Dissolve dried samples with 0.1M HNO3.
3) Adjust pH between pH2 and 3 by NaOH solution.
4) Apply to Ca assay kit (dilution will be needed).
|
| 3 |
Stool samples: |
1) Homogenate stool sampls into powder or small particles.
2) Mix 0.1g of homogenated stool sample with 1mL of 0.1 M of HCl, and shake for 30 minutes.
3) Centrifuge 10,000 rpm for 10 min at 4°C, and collect supernatant.
4) Apply to Ca assay kit (For dilution, please use 0.1 M HCl).
Extraction will be time dependent. Please take care that the extraction time are to be same for all samples.
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Ask a question about Ca / Calsium Assay Kit (o-Cresolphtalein-Complexone) by e-mail.
|
|
|
| List of references: |
| 1) |
Fujita T, Noguchi K, Terashima I:
Apoplastic mesophyll signals induce rapid stomatal responses to CO2 in Commelina communis.
New Phytol 199(2),p395-406(2013). doi: 10.1111/nph.12261
|
| 2) |
Kenta Maruyama, Satoshi Uematsu, Takeshi Kondo, Osamu Takeuchi,
Mikael M. Martino, Takumi Kawasaki, Shizuo Akira:
Strawberry notch homologue 2 regulates osteoclast fusion by enhancing the expression of DC-STAMP.
J Exp Med 210(10),p1947-1960(2013) doi: 10.1084/jem.20130512
|
| 3) |
Hisano K, Fujise O, Miura M, Hamachi T, Matsuzaki E, Nishimura F:
The pga gene cluster in Aggregatibacter actinomycetemcomitans is necessary for the development of
natural competence in Ca(2+) -promoted biofilms.
Mol Oral Microbiol 29(2),p79-89(2014). doi: 10.1111/omi.12046
|
| 4) |
Kenji Okano, Shimpei Miyamaru, Ayaka Kitao, Hiroyuki Takano,
Tsuyoshi Aketo, Masaya Toda, Kohsuke Honda, Hisao Ohtake:
Amorphous calcium silicate hydrates and their possible mechanism for recovering phosphate from wastewater.
Separation and Purification Technology 144,p63-69(2015)
http://dx.doi.org/10.1016/j.seppur.2015.01.043
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| 5) |
Takashi Kuribayashi, Keigo Sato, Toshio Joh, Mitsuoki Kaneoke, Ken-ichi Watanabe:
Simple differentiation of sake (Japanese alcoholic beverage) based on trace inorganic components using colorimetric methods.
Journal of Bioscience and Bioengineering 121(1),p43-46(2016)
http://dx.doi.org/10.1016/j.jbiosc.2015.05.004
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| 6) |
Daisuke Nakamura, Makoto Yoshimitsu, Ayako Kuroki, Miho Hachimana, Yuhei Kamada, Chibueze C. Ezinne,
Akihiko Arai, Hirosaka Inoue, Heiichirou Hamada, Maiko Hayashida, Shinsuke Suzuki, Satoshi Fujino, Naosuke Arima, Mamiko Arima,
Tomohisa Tabuchi, Seiji Okada, Naomichi Arima:
A new ATL xenograft model and evaluation of pyrrolidine dithiocarbamate as a potential ATL therapeutic agent.
Experimental Hematology 43(11),p944-950(2015)
http://dx.doi.org/10.1016/j.exphem.2015.06.303
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| 7) |
Kenta Maruyama, Takahiko Kawasaki, Masahide Hamaguchi, Motomu Hashimoto, Moritoshi Furu,
Hiromu Ito, Takao Fujii, Naoki Takemura, Thangaraj Karuppuchamy, Takeshi Kondo, Takumi Kawasaki, Masahiro Fukasaka,
Takuma Misawa, Tatsuya Saitoh, Yutaka Suzuki, Mikael M. Martino, Yutaro Kumagai and Shizuo Akira:
Bone protective functions of Netrin 1.
Journal of Biological Chemistry (2016)
http://dx.doi.org/10.1074/jbc.M116.738518
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Ask a question about Ca / Calsium Assay Kit (o-Cresolphtalein-Complexone) by e-mail.
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| List of references: |
| 1) |
Akanuma G, Kobayashi A, Suzuki S, Kawamura F, Shiwa Y, Watanabe S, Yoshikawa H,
Hanai R, Ishizuka M:
Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.
J Bacteriol 196(22),p3820-3830(2014) doi: 10.1128/JB.01896-14
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| 2) |
Sakamoto A, Terui Y, Yamamoto T, Kasahara T, Nakamura M, Tomitori H, Yamamoto K,
Ishihama A, Michael AJ, Igarashi K, Kashiwagi K:
Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators
UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor.
Int J Biochem Cell Biol 44(11),p1877-1886(2012). doi: 10.1016/j.biocel.2012.07.010
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Ask a question about Mg / Magnesium Assay Kit (Xyridil Blue-I) by e-mail.
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